Identification of AHL Synthase in Desulfovibrio vulgaris Hildenborough Using an In-Silico Methodology

Sulfate-reducing bacteria (SRB) are anaerobic that form biofilm and induce corrosion on various material surfaces. The quorum sensing (QS) system employs acyl homoserine lactone (AHL)-type QS molecules primarily govern formation. Studies SRB have reported the presence of AHL, but no AHL synthase been annotated in so far. In this computational study, we used a combination data mining, multiple sequence alignment (MSA), homology modeling docking to decode putative model SRB, Desulfovibrio vulgaris Hildenborough (DvH). Through shortlisted 111 genes. Conserved domain analysis genes generated consensus sequence. Subsequent MSA with DvH genome indicated DVU_2486 (previously uncharacterized protein from acetyltransferase family) is gene encoding for synthase. Homology revealed existence seven α-helices six β sheets amalgamated study hydrophobicity, binding energy, tunnels cavities Leu99, Trp104, Arg139, Trp97, Tyr36 crucial amino acids catalytic center Identifying would provide more comprehensive knowledge mechanism help design strategies control